贵州喀斯特地区碳酸盐岩表生古菌群落结构及多样性研究——以南江大峡谷为例

为了研究贵州喀斯特地区碳酸盐岩表生古菌群落结构的多样性,应用16SrDNA文库技术,对采集于贵州南江大峡谷的白云岩和石灰岩样品进行基因文库的构建和限制性片段长度多态性(RFLP)分析。用限制性核酸内切酶Msp I对两个文库中分别随机挑选的300个阳性克隆子进行酶切分型,白云岩和石灰岩16SrDNA基因文库各得到14和13个基因型,其覆盖率分别为95.4%和91.3%,香农指数分别为2.14和1.93。系统发育分析表明白云岩和石灰岩表生古菌克隆子全部归属于泉古菌门(Crenarchaeota),代表性克隆与Gen Bank数据库已有16S rRNA序列的相似性为96%~100%,且最高相似性序列...

Full description

Saved in:
Bibliographic details
Volume: 36
Main Author: 唐源 连宾 程建中
Format: Journal Article
Language: Chinese
Place of publication: 中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳,550081%南京师范大学生命科学学院,南京,210023 2017
published in: Zhongguo yan rong Vol. 36; no. 2; pp. 193 - 201
Data of publication: 2017
ISSN: 1001-4810
Alternate Title: Archaeal community structure and diversity of the carbonate rocks in karst regions, Guizhou:A case study of the Nanjiang canyon
Classification Codes:
Discipline: Geography
Bibliography: dolomite, limestone, archaea, diversity, karst
45-1157/P
TANG Yuan1 , LIAN Bin2 , CHENG Jianzhong1(1.State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang ,Guizhou 550081, China ; 2. School of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210023, China)
Guizhou is one of the three largest karst regions in the world where vast expanses of karst develop well. The carbonate rock area is 13 × 104 km2 , covering 73% of total land surface area of the province. Car- bonate rock is very challenging for organisms to live in it owing to its features, such as arid environment, nu- tritional deficiency, and great temperature fluctuations. However, there are various microorganisms on its surface and in its cracks, which play a crucial role in biogeochemical cycle. The Nanjiang canyon is a typical karst canyon, which is praised as "Karst Ecosystem Museum". It is located in Kaiyang County of Guizhou Province, southwestern China. And there are a lot of ex
Subjects:
Online Access: available in Bonn?
Database: 中文科技期刊数据库
中文科技期刊数据库-CALIS站点
中文科技期刊数据库-7.0平台
中文科技期刊数据库-自然科学
中文科技期刊数据库- 镜像站点
Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
Database information Databases - DBIS